Difference between CDS and cDNA - Biology Stack Exchange
Nov 8, 2013 · What is the difference between Coding Sequences (CDS) and cDNA? Are Coding sequences the sequences that is transcribed to mRNA and cDNA in contrast DNA obtained by …
Complementarity of cDNA - Biology Stack Exchange
Strictly speaking, what is the definition of cDNA? This confuses me, since usually it is said to refer to DNA that is complementary to mRNA. Is this correct? Is it restricted to mature mRNA? I ...
Minimum amount of RNA for cDNA conversion and qPCR
Nov 28, 2019 · I searched several protocols for reverse transcription of RNA and all suggest using RNA concentrations of 1ug. Could a lower amount result in inferior transcript representation …
Reverse transcription PCR optimization - Biology Stack Exchange
Jan 12, 2012 · What is the ideal amount of RNA to use for the RT? and how much cDNA to use then for the PCR? I did RT with a solution of RNA of 0.36 ug/ul. Then for my PCR I used 1 ul …
Why use DNA polymerase in making cDNA? - Biology Stack …
Nov 17, 2013 · RT is capable of synthesizing a complementary dna strand ( as in HIV life cycle.) Then why is DNA pol used when cDNA (synthesizing the second strand of it ) has to be …
Large scale reverse transcription? - Biology Stack Exchange
I need to make RNA:DNA duplexes. I can make 100 to 200 ug of mRNA through in vitro transcription, and I know how to use reverse transcription to make a cDNA library, but I have …
What are Expressed Sequence Tags (EST)? - Biology Stack Exchange
Mar 25, 2017 · I read in an article that ESTs are partial or sub-sequences of cDNA. What are ESTs exactly? Which part of cDNA is cut in order to make ESTs? Why are they so …
Storing Taqman plates with polymerase in fridge before running
May 10, 2015 · @MarchHo the cDNA has been prepared ahead of time and frozen. On the day of the qPCR, I prepare the reaction using 2 dye-labeled probes (gene of interest and internal …
Open Reading Frames vs Coding Sequences (CDS), are they …
Mar 5, 2020 · I (mostly) like the definitions given on this page. An ORF is a sequence of DNA that starts with start codon “ATG” (not always) and ends with any of the three termination codons …
Why is the GenBank entry for the genomes of RNA viruses like ...
Apr 5, 2020 · RNA is sequenced by reverse transcribing it to cDNA, and this is the sequence that is submitted. In fact, I don't think I've ever seen a sequence in NCBI specifying uracil instead …